Journal: iScience

Article Title: High-throughput longitudinal electrophysiology screening of mature chamber-specific hiPSC-CMs using optical mapping

doi: 10.1016/j.isci.2023.107142

Figure Lengend Snippet:

Article Snippet: Human ECM-coated cell culture plates (CELLvo MatrixPlus, StemBioSys, Inc.) promoted significant maturation of hiPSC-CM toward rod-shaped cells reminiscent of cardiomyocytes isolated from adult mammalian hearts ( and ). hiPSC-CM sarcomere length was longer and more organized in cells maintained on MatrixPlus-human ECM ( C and 1D).

Techniques: Virus, Plasmid Preparation, Recombinant, Membrane, Isolation, Software

Journal: PLoS ONE

Article Title: CD44 Upregulation in E-Cadherin-Negative Esophageal Cancers Results in Cell Invasion

doi: 10.1371/journal.pone.0027063

Figure Lengend Snippet: (A) Western Blot analysis of esophageal squamous carcinoma cell lines (TE-1 -7, -8, -11, and -12), esophageal adenocarcinoma cell lines (FLO-1, OE33, SK-GT-4) and head and neck cancer cell lines (JHU-012, JHU-013) show that expression of E-cadherin and TGFβRII is associated with low levels of CD44, while loss of E-cadherin and TGFβRII correlates with up-regulation of CD44 and the EMT marker vimentin. (B) Restoration of E-cadherin, Ecad, expression by retroviral transfection of TE-8 and FLO-1 cells compared to vector control (neo) demonstrates an overall decrease in CD44 expression along with a switch to increased CD44v over CD44s (CD44v: CD44variant, CD44s: CD44 standard).

Article Snippet: After 24 hours, medium was changed to serum-free DMEM and cells were transfected with a final concentration of 10 nM siRNA using calcium phosphate transfection (400 μL of RNAse-free water was mixed with 50 μL 2.5 M CaCl 2 , 50 μL of 2 μM siRNA with 500 μL of HBSP2x (280 mM NaCl, 1.5 mM NA 2 PO 4 2H 2 O, 12 mM glucose, 10 mM KCL, 50 mM HEPES pH7.05 in 500 mL RNAse-free water)). siRNA were obtained from Qiagen (Qiagen, Valencia, CA), Hs-CDH1_12 (si1) and Hs_CDH1_13 (si2) were used against E-cadherin and AllStars negative, non-silencing siRNA probes were used for control (ctrl).

Techniques: Western Blot, Expressing, Marker, Retroviral, Transfection, Plasmid Preparation, Control